Figure 4

Immunolocalisation of ghrelin and GHSR-1a in the testes from adult sheep maintained in either short- or long-day photoperiods. Photomicrographs of adult sheep testicular sections kept for 12 weeks in either a short day (SD) or long day photoperiod (LD). (A) Section from a SD sheep showing positive staining for ghrelin in the interstitium (INT), Sertoli cells (SC), pre-spermatogonia (PS) and round spermatids (RS). (B) Section from a LD sheep showing reduced staining for ghrelin in the testis compared to the SD sheep. (C) Section from a SD sheep showing positive staining for GHSR-1a. (D) Section from a LD sheep showing reduced staining for GHSR-1a in the testis compared to the SD sheep. (E) The scale bars of A, B, D, E represent 50 μm.