Figure 3
Immunohistochemical and in situ localisation of ghrelin and GHSR-1a in sheep gonads. Photomicrographs of sheep ovarian and testicular sections. (A) Ovarian section showing positive immunostaining for ghrelin in the stroma, primordial follicles (PF) and the ovarian surface epithelium (OSE). (B) Ovarian section showing positive immunostaining for ghrelin in secondary follicles (SF) and granulosa cells (GC). (C) High magnification micrograph showing positive immunostaining for ghrelin in the corpus luteum (CL). (D) Ovarian section showing positive immunostaining for GHSR-1a in the stroma, PF and OSE. (E) Ovarian section showing positive immunostaining for GHSR-1a in the SF and GC. (F) High magnification micrograph showing positive immunostaining for GHSR-1a in the CL. (G) Testicular section of seminiferous tubules showing positive immunostaining for ghrelin in the Sertoli cells (SC), pre-spermatogonia (PS), round spermatids (RS) and in the interstitium (INT). (H) Testicular section of seminiferous tubules showing positive immunostaining for GHSR-1a in the SC, PS, RS and INT. (I) Autoradiograph of adult testis sections following in situ hybridisation to an antisense 35S-labelled riboprobe to GHSR-1a mRNA showing hybridisation mainly in the interstitial areas between the seminiferous tubules. The scale bars of A, B, D, E, I represent 50 μm, and for C, F, G, H they represent 150 μm The insert is the negative control (in situ sense control for I).