Skip to main content

Advertisement

Table 1 Properties of oligonucleotide primers used for PCR.

From: Extragonadal aromatization increases with time after ovariectomy in rats

Target mRNA Size of PCR products (bp) Sense (S) or antisense (AS) Primer sequence (location)
P450c17 299 S
AS
5'-TCATCAAGAAGGGAAAAGAA-3'
5'-TGAAGCAGATAGCACAGATG-3'
P450arom 289 S
AS
5'-GCTTCTCATCGCAGAGTATCCGG-3'
5'-CAAGGGTAAATTCATTGGGCTTGG-3'
β-actin 550 S
AS
5'-AAGCAGGAGTATGACGAGTCCG-3'
5'-GCCTTCATACATCTCAAG TTGG-3'