Skip to main content

Table 1 Primer sequences of 18SrRNA and IGF-I for real-time PCR and plasmid construction (†).

From: Easy and rapid method for the determination of gene expression in cumulus cells incubated for oocyte maturation

Genes Accession No. Primer sequences
18SrRNA AF176811 5'-AACGGCTACCACATCCAA-3' 5'-GACTCATTCCAATTACAGGGC-3'
   5' – TGACGGGGAATCAGGGTT-3' (†) 5' – CAAAGTAAACGCTTCGGGC-3' (†)
IGF – I X15726 5'-GGA GTG CAG GAA ACA AGA AC-3' 5'-TTT TGG TAG GTC TTC TGG TG-3'