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Table 1 Primer sequences of 18SrRNA and IGF-I for real-time PCR and plasmid construction (†).

From: Easy and rapid method for the determination of gene expression in cumulus cells incubated for oocyte maturation

Genes

Accession No.

Primer sequences

18SrRNA

AF176811

5'-AACGGCTACCACATCCAA-3'

5'-GACTCATTCCAATTACAGGGC-3'

  

5' – TGACGGGGAATCAGGGTT-3' (†)

5' – CAAAGTAAACGCTTCGGGC-3' (†)

IGF – I

X15726

5'-GGA GTG CAG GAA ACA AGA AC-3'

5'-TTT TGG TAG GTC TTC TGG TG-3'