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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Easy and rapid method for the determination of gene expression in cumulus cells incubated for oocyte maturation

Figure 4

Amplification curves (A) and melting curves (B) of 18SrRNA amplified by real-time PCR using the RT-minus and plus products of the cell lysate (certified residual DNA contamination-free) at 2 mM MgCl2, and gel-electrophoresis pattern of PCR product (C). Lane 1: 100-bp Ladder size marker; Lane 2: amplification product of RT-minus (60 bp). Lane 3:. amplification product of RT-plus (120 bp).

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