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Table 2 Candidate molecules identified (NCBInr) from proteins immunopurified from G/LCM using immobilised phage-derived IgG and rat pAb.

From: Selection of gonadotrophin surge attenuating factor phage antibodies by bioassay

Immuno-purification Ab

Spot/band

Database

Protein

MOWSE Score

Accession Number

  

PI

kDa

   

rpAb

1-D 66 kDa band

5.9

69.2

Serum albumin precursor

224,400

P02768

 

Excl albumin peaks

9.08

86.8

SAD1 kinase

767

Q8TDC3

 

2-D #1

6.0

56.8

PRO2619

372,800

11493459

  

5.9

69.2

Serum albumin precursor

228,770

6013427

 

#2

5.8

72.8

Solute carrier family 39, member 12

1,855

22749433

 

#3

5.0

57.2

Keratin 10

6,260

88041

 

#5

6.0

56.8

PRO2619

102,500

11493459

  

5.9

69.2

Serum albumin precursor

78,270

6013427

 

#6

6.0

56.8

PRO2619

2,067,000

11493459

  

5.9

69.2

Serum albumin precursor

1,302,000

6013427

Derived IgG

1-D 74 kDa band

  

Insufficient protein

  
 

2-D #4

5.9

69.2

Serum albumin precursor

2.483e+11

6013427

 

#7

5.9

69.4

Hypothetical protein

6.366e+11

51476390

  

5.9

69.2

Serum albumin precursor

2.276e+11

6013427