Figure 1

Diagram showing the strategy to identify GnSAF-recognising scAbs. (1) 96-well dishes were coated with secondary antibodies that would and would not bind the scAbs/IgGs and then (2) incubated with candidate anti-GnSAF and control scAbs/IgGs. When (3) GnSAF-containing QC hFF or G/LCM were added, immobilised GnSAF-recognising scAbs/IgGs bound some or all the GnSAF so that when the medium was (4) removed from the wells and added (5) to our rat pituitary monolayer bioassay, GnSAF bioactivity (6) would be reduced. Where the sequence of secondary and primary antibodies did not include the appropriate coating antibody and GnSAF-recognising scAb/IgG, GnSAF bioactivity would not be significantly reduced.