Figure 9
Confocal scanning laser micrographs of in vivo matured metaphase II oocytes labeled with either anti-PAD V (N) (A – D) or ABL2 (E – H). showing that PAD is a peripheral membrane protein. A and E are artificially activated oocytes showing released PAD and ABL2 antigen (p75) on the oocyte's surface. B and F are artificially activated oocytes treated with a high salt solution that removed PAD and p75 from oocytes' surfaces. C and G are DMSO control oocytes showing PAD and ABL2 antigen in cortical granules. D and H are DMSO control oocytes treated with a high salt solution confirming that PAD and ABL2 antigen were still detected in cortical granules.