Figure 6

Confocal scanning laser micrographs comparing distribution of PAD and LCA-binding cortical granule components in in vivo fertilized oocytes and in vivo matured 2-cell embryos. Optical sections (A – C and G – I) and two-dimensional projections of z-series (D – F and J – L) of zona intact fertilized oocytes (A – F) and zona intact 2-cell embryos (G – L) labeled with LCA (red) and ABL₂ antibody (green). A, D, G, and J show the LCA labeling on the surface of the oocyte (white arrowhead), in the perivitelline space (arrow), and in the zona pellucida (yellow arrowhead). B, E, H, and K show the ABL₂ labeling. C, F, I, and L are merged confocal images and two-dimensional projections showing both LCA and ABL₂ labeling.