Figure 1
Tissue distribution of the ABL2 antigen. (A) Silver-stained SDS-PAGE gel loaded with the ABL2 immunoprecipitate from mouse brain (lane 1), liver (lane 2), skeletal muscle (lane 3), ovary (lane 4), oviduct (lane 5), and testis (lane 6). The ABL2 antibody immunoprecipitated a 75-kDa protein from the ovarian lysate but not from other tissues. Other bands in the gel are from the antibody used for immunoprecipitation. (B) Confocal scanning laser micrographs of germinal vesicle intact mouse oocytes double labeled with the lectin LCA (LCA) and the ABL2 antibody (ABL2). The merged image (LCA + ABL2) showed co-localization of LCA and ABL2 in some cortical granules. These images were digitally enlarged for better visualization. (C) Western blots in which ABL2 immunoprecipitate was probed with the lectins ConA, LCA, WGA, PNA, and DBA. Control blots were probed with lectins preabsorbed with the appropriate control sugar. Positive controls (blots with rabbit IgG) were included for each lectin to show that the blotting condition was optimized.