Figure 9
Competition curves for the binding of various natural steroids to AR in kidney. A, cytosolic extracts, and B, reticulocyte lysate samples. Varying concentrations of unlabelled steroids were incubated with 1 nM [3H]-DHT over night. Each data point represents the average of two assays. Steroids used were: KT, 11-ketotestosterone; DHT, 5α-dihydrotestosterone; T, testosterone; E2, 17β-estradiol; 17,20β-P, 17,20β-dihydroxy-4-pregnen-3-one; 20β-S, 17,20β,21-trihydroxy-4-pregnen-3-one; F, cortisol.