Figure 6
In vitro translation products of three-spined stickleback ARβ1 and β2. AR was expressed by introducing the pCMV-TNT into the TNT Coupled Reticulocyte Lysate System. The size of the expressed products was determined by addition of S35-methionin to the reticulocyte lysate system. The protein suspensions were analyzed on a 8% SDS PAGE. Deduction of protein size from sequencing data yielded a expected size of 76.6 kDa for ARβ1 and 82.2 kDa for ARβ2. Estimation of translation product sizes resulted in 61 kDa and 52 kDa for ARβ1 and 87 kDa and 75 kDa for ARβ2.