Figure 5

Sequence alignment of the ligand-binding region of AR. The alignment was performed using Omiga 2.0 (Oxford Molecular Ltd). Amino acid identity between sequences was illustrated with grey boxes and amino acid match with black boxes. Numbering is according to the human AR sequence. Amino acids (N705, Q711, R752, T877) with possible interaction with DHT in human AR are indicated by arrows from above. Amino acids with possible close contact to DHT (L704, M745 and F764) and of importance for ligand binding pocket architecture (R779) are indicated with arrows from below. GenBank Accession Nos.: Human AR (hAR) (M34233); Mouse AR (mAR) (X53779); Zebra finch AR (zAR) (AF532914); African clawed toad AR (aAR) (U67129); Tilapia ARα (tARα) (AB045211); Tilapia ARβ (t ARβ) (AB045212); Japanese eel ARα (jARα) (AB023960); Japanese eel ARβ (jARβ) (AB025361); Red seabream AR (rAR) (AB017158); Three-spined stickleback ARβ (sAR) (AY247207).