Figure 5

Gene expression of IFN-γα and β chain receptor mRNA in cultured ectoplacental cones. (A) A representative RT-PCR analysis of mRNA transcript in ectoplacental cone outgrowth cultures lacking (lanes 2 to 4) or with (lanes 5 to 7) mrIFN-γ. Lane 1 represents the DNA ladder, lanes 2 and 5, cyclophilin, used as an internal cDNA control (668 bp), lanes 3 and 6, IFN-γ receptor α chain (608 bp) and lanes 4 and 7, IFN-γ receptor β chain (529 bp). (B) Semi-quantitative RT-PCR analysis of IFN-γ receptor chain expression. A summary of 3 experiments is shown in the bar graph (mean and S.D.). Transcripts were expressed as a ratio between band intensities of the IFN-gamma receptor chain and cyclophilin. Asterisk shows statistical differences in relation to the control (c) in which no IFN-γ has been added (p < 0.05). Numbers represent: 1 and 2, IFN-γ α chain receptor expression in the absence (c) and presence of IFN-γ in the culture medium, and 3 and 4, IFN-γ β chain receptor expression in the absence (c) and presence of IFN-γ.