Figure 1

Forty eight hour-cultured ectoplacental cone cells. Samples were stained for cytokeratin-A intermediate filaments typical of trophoblast cells (A-C) or YO-PRO to localize cell death (D-I). IFN-γ treatments were: D-E, no treatment;F-G, 100 U/mL and,H-I, 10,000 U/mL. The arrows in figure C highlight cytoplasmic filaments and in figures D-I, dead cells. A-C, Alkaline phosphatase immunostaining. B, Negative control in which the primary antibody was omitted. D, F and H, Fluorescence microscopy. E,G and I, Nomarski imaging of D,F and H, respectively. The unique bar in figure A represents: 300 μm in A, 160 μm in B, and 80 μm in C.