Figure 2

Flow cytometric analyses of sperm from hemizygotes for an overexpressed or a Spam1 null allele show bimodal distributions. a) Caudal sperm from the congenic wild-type, Tg11/+ and Tg8/+ show a bimodal distribution only for Tg8/+. The second peak (on the right) with the greater intensity in this bimodal distribution indicates the presence of a subpopulation of sperm with overexpression. The distributions for the wild-type and Tg11/+ are unimodal with lower mean intensities, indicating a lack of Spam1 overexpression. b) Caudal sperm from Tg8 carriers showing the analysis and gating of the sperm for sorting. c) Caput sperm from hemizygous null mice show a bimodal distribution in sperm in A and B. The first peak (on the left) in each shows background levels of fluorescence, likely representing sperm with the null allele.