|
Jauniaux et al [59]
|
Immunohistochemistry
|
Heat shock protein 70, hydroxynenal, nitrotyrosine residues.
|
Fluorescence intensity
|
|
Wang et al, Djordjevic et al [60, 61]
|
Antioxidant enzyme activity assays
|
Total SOD activity, catalase activity, glutathione peroxidase activity, reduced glutathione assay
|
Change in optical density/minutes/mg protein
|
|
Wiktor et al [62]
|
Oxidative DNA adducts
|
8-hydroxy-2 deoxyguanosine
|
Micromoles/mole of 2-deoxy guanosine
|
|
Holthe et al [63]
|
Superoxide anion, hydrogen peroxide and peroxynitrite
|
Dihydrethidium probe Dichlorodihydrofluorescein Dihydrorhodamine 123 Spectrophotometry/flow cytometry
|
Nanomoles/10 min/106 cells
|
|
Ishihara et al [64]
|
Lipid peroxidation products
|
Isoprostane, Urinary 8-epi-prostaglandin F2lpha, assayed by gas chromatography/mass spectrophotometer analysis
|
pg/mg of creatinine
|
|
Vaisanen-Tommiska et al [65]
|
Nitric oxide; Greiss reaction
|
Stable end products: nitrite/nitrate
|
Fluorometric assay, results expressed as NOx (sum of converted nitrite and very small amount of nitrite in serum).
|
|
Buhimishi et al [66]
|
Plasma and red blood cell glutathione content
|
Colorimetric assay
|
Nanomoles/mgm of haemoglobin
|
