Table 1 PCR primer and Taq-Man® detection probes sequences for interrogation of AT1, AT2, GAPDH by qRT-PCR.
Primer/Probe | Sequence |
|---|---|
GAPDH-1 | GCA AGT TCC ACG GCA CAG T |
GAPDH-2 | GGT GAT GGC CTT TCC ATT GA |
GAPDH-det | (6FAM)AAG GTC AGA AAA CGG GAA GCT CGC TC(MGBNFQ) |
AT2R-1 | CAT GGA AGG GAA GCC AAC AA |
AT2R-2 | ACT CGT GAC CAA GTT CTG AAG ATG |
AT2R-det | (VIC)TGA TGA ACG CCA GAA CAA CAG CAG C(MGBNFQ) |
AT1U604 | CCC TTC GGC AAT TAC CTA |
AT1L682 | GCG GTC AAT GCT TAG ACA |