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Table 2 Half-lives of RS cocultured with Sertoli cells in the absence or presence of TGFβ1

From: Transforming growth factor beta-1 decreases the yield of the second meiotic division of rat pachytene spermatocytes in vitro

Treatment

Half-life (days)

Control

1.9 ± 0.2

TGFβ1

1.9 ± 0.2

  1. Elutriated RS were cocultured with Sertoli cells for 5 days in the absence (control) or presence of 10 ng/ml TGFβ1 and the number of remaining RS was assessed by flow cytometry daily between day 2 and day 5 allowing the determination of their half-life in culture. Each value is the mean ± SEM of three different experiments.