- AR mRNA and protein expression. The expression of β2-AR mRNA in the ovary (panel A) in the PCO group was lower compared to the control group. β2-AR mRNA was unaltered in the PCO+EA group when compared to control. Values are given as means ± SEMs normalized to GAPDH. ap < 0.05 vs control group. β2-AR immunopositive cell number (Panel B) in control ovaries was unchanged by EA treatments. No difference in number of β2-AR immunoreactive cells was found in PCO ovaries, while EA treatments in PCO rats significantly increase the amount of β2-AR immunostained cells when compared to PCO group. Values are given as means ± SEMs. bp < 0.05 vs PCO group. Representative pictures of stained cells (some of them pointed by black arrows) are showed in Panels C- D. The β2-AR was found expressed in degenerating corpora lutea (C) and follicles (D) in all of the experimental groups. Magnification C: ×400; D: ×200.