Figure 5

Effect of anti-α6 IgG on morphology of GC cultured on substrata containing different ratios of LN and RGD peptides. GC were cultured for 48 h on substrata containing percentages of LN varying between 100% (0% RGD peptides) and 0% (100% RGD peptides) in the mix, with or without anti-α6 IgG (0.5 μg/ml) in culture medium, and then actin was stained with FITC-conjugated phalloidin. (a) and (b): representative microscopic fields of cultured GC from small (a) and large (b) follicles. (c) and (d): percentages of round GC from small (c) and large (d) follicles; empty bars: control; solid bars: with anti-α6 IgG in culture medium; data represent mean ± SEM of 5 independent experiments; * : p < 0.05, ** : p < 0.01, *** : p < 0.001, with vs. without anti-α6 IgG.