Effect of cytochalasin D on cell numbers, proliferation and pyknotic rates of GC cultured on LN substratum. GC from small (a, c) and large follicles (b, d) were cultured for 144 h on LN as described in legend of Figure 2. (a) and (b): numbers of cells at 144 h of culture; (c): proliferation rates of GC from small follicles at 48 h of culture; (d): pyknotic rates of GC from large follicles at 48 h of culture. Empty bars: control; solid bars: with anti-α6 IgG (0.5 μg/ml) in culture medium. Data represent mean ± SEM of 6 independent experiments. In each graph, different letters indicate significant differences (p < 0.05); * : p < 0.05, *** : p < 0.001, with vs. without anti-α6 IgG.