Effect of anti-α6 IgG on morphology and functions of GC cultured on LN substratum. GC were cultured up to 144 h on LN with (b and solid bars in c, d, e and f) or without (a and empty bars in c, d, e and f) anti-α6 IgG (0.5 μg/ml) in culture medium. (a) and (b): representative microscopical fields of cultured GC throughout the culture period; (c): proliferation rates of GC from small follicles, assessed by thymidine labelling at 48 h of culture; (d): pyknotic rates of GC from large follicles at 48 h of culture; (e): progesterone (P4) secretion by GC from large follicles between 96 h and 144 h of culture; (f): estradiol (E2) secretion by GC from large follicles between 96 h and 144 h of culture. Data represent mean ± SEM of 5 independent experiments. * : p < 0.01, with vs. without anti-α6 IgG.