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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Oogenesis in cultures derived from adult human ovaries

Figure 1

Cell phenotypes in 6-day OSE culture maintained in phenol red free DMEM/F12 medium. a) Live fibroblast type cells in phase contrast microscopy (PhC) show perinuclear accumulation of organelles (arrowhead). b), Immunostaining of the same cells with HSPZ antibody shows ZP+ nuclei and perinuclear organelles (arrowhead). c) A cluster of cells showing epithelial phenotype with ZP+ nuclei and perinuclear organelles (arrowhead). d) ZP+ neural type cell shows extensions toward the ZP+ perinuclear staining (arrowhead) of neighboring mesenchymal type cells. Inset shows similar type of cell in the live culture. e) Clusters (arrows) of small (15 μm) cells of granulosa cell phenotype with strong ZP expression. Arrowhead indicates mesenchymal type cells. f) Double staining for ZP/CK5,6,8,17 (blue substrate) shows dark-blue staining of small cell clusters (arrows), but mesenchymal type cells remain brown only (arrowhead).

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