(Top panel, a) Immunofluorescence analysis of histologic cryosections of seminiferous tubules cultured for 6 h and immunostained with antibodies raised against clusterin. 1. control (Bar 25x); 2. MAA (Bar 10x); 3. MAA plus ω-agatoxin (Bar 25x). Single arrow indicates pachytene spermatocytes; double arrow indicates Sertoli cells. Representative data of n = 3 independent experiments. (Lower panel, b) Quantitative analysis by flow cytometry of testicular cell population prepared from seminiferous tubules cultured for 6 h. Grey bar represents clusterin content (p < 0.001). Black bar represents apoptosis levels determined by TUNEL. Data are expressed as mean +/- SEM of three independent experiments. 1. control; 2. MAA; 3. MAA plus ω-agatoxin.