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Table 1 List of primers and conditions employed for PCR

From: Effects of ICI 182780 on estrogen receptor expression, fluid absorption and sperm motility in the epididymis of the bonnet monkey

Gene

Primer Sequence

Annealing Temp (°C)

Product size (base pairs)

ERα

FP 5' gag aca tga gag ctg cca ac 3'

RP 5' cca aga gca agt tag gag ca 3'

55

381

AQP-1

FP 5' cag cat ctt ccg tgc cct cat gta 3'

RP 5' cat act cct cca cct ggc cgc tgg 3'

58

495

Na+-K+ ATPase-α1

FP 5' aaa ctt agc ctt gat gag ctt 3'

RP 5' tcc atg atc ttt gaa ctt tta 3'

55

344

GAPDH

FP 5' gga gtc aac gga ttt ggt 3'

RP 5' gtg atg gga ttt cca ttg at 3'

54

206

  1. FP: Forward Primer
  2. RP: Reverse Primer