Skip to main content

Table 1 List of primers and conditions employed for PCR

From: Effects of ICI 182780 on estrogen receptor expression, fluid absorption and sperm motility in the epididymis of the bonnet monkey

Gene Primer Sequence Annealing Temp (°C) Product size (base pairs)
ERα FP 5' gag aca tga gag ctg cca ac 3'
RP 5' cca aga gca agt tag gag ca 3'
55 381
AQP-1 FP 5' cag cat ctt ccg tgc cct cat gta 3'
RP 5' cat act cct cca cct ggc cgc tgg 3'
58 495
Na+-K+ ATPase-α1 FP 5' aaa ctt agc ctt gat gag ctt 3'
RP 5' tcc atg atc ttt gaa ctt tta 3'
55 344
GAPDH FP 5' gga gtc aac gga ttt ggt 3'
RP 5' gtg atg gga ttt cca ttg at 3'
54 206
  1. FP: Forward Primer
  2. RP: Reverse Primer