Figure 6

Change in the luminal diameter of efferent ductules after in-vitro incubation with ICI: Efferent ductules were minced and tissue fragments of 2–3 mm were incubated in M199 medium containing dihydrotestosterone (1 nM), 17β-estradiol (1 nM) with or without ICI (1 μM) for 24 hr. The ends of the tubules were ligated with a sterile catgut and incubated in a fresh medium of the same composition, for further 24 hr. Subsequent to this total incubation period of 48 hr, the ductules were rapidly fixed in Bouin's fluid and embedded in paraffin. Sections were stained in hematoxylin and eosin and diameter of the tubules measured. In absence of ICI, the control (Panel A) shows reduced luminal diameter compared to ICI-incubated efferent ductules (Panel B). Panel C is a graphical representation of the luminal diameters. Values represent Mean ± SEM from three independent experiments. Bar in Panel A = 100 μ; *** P < 0.001.