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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Effects of ICI 182780 on estrogen receptor expression, fluid absorption and sperm motility in the epididymis of the bonnet monkey

Figure 4

a-b RT-PCR analysis for ERα, AQP-1, Na+-K+ ATPase-α1 and GAPDH in the caput regions of control and 30-day (Fig 4a) and 60-day (Fig 4b) ICI treated monkeys: RNA isolated from the caput region of vehicle-and ICI-treated bonnet monkeys, was reverse transcribed and the cDNA was subjected to semi-quantitative PCR in the linear range of amplification with GAPDH amplification as an internal control. The intensity of ERα expression (Panel A) increased upon ICI treatment compared to that of vehicle-treated controls, with the increase being appreciably greater in the 30-day treatment period. AQP-1 levels were reduced in the ICI-treated group compared to controls (Panel B). There was no discernable change in the Na+-K+ ATPase-α1 levels between the ICI-treated groups and vehicle controls (Panel C). Panel E is a graphical representation of this data wherein, the densitometric signal intensities obtained for ERα, AQP-1 and Na-K ATPAse-α1 were normalized to that of GAPDH (Panel D). Data represents Mean ± SEM of three experiments. **P < 0.05, ***P < 0.001.

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