Figure 1

a RT-PCR analyses of ERα (Panel A), AQP-1 (Panel B), Na⁺-K⁺ ATPase-α1 (Panel C) and GAPDH (Panel D) in the bonnet monkey epididymis: RNA isolated from the caput, corpus and cauda regions of the bonnet monkey epididymis was reverse transcribed and the cDNA so obtained was subjected to semi-quantitative PCR in the linear range of amplification with GAPDH amplification as an internal control. Panel E is a graphical representation of this data wherein, the densitometric signal intensities obtained for ERα, AQP-1, Na⁺-K⁺ ATPase-α1 were normalized against that of GAPDH and plotted as percent change of the individual intensities with respect to the caput region. Data represents Mean ± SEM of three independent experiments. **P < 0.05, ***P < 0.001. b Western blot analyses for ERα and ERβ in the bonnet monkey epididymis: 100 μg each of protein lysates obtained from caput, corpus and cauda regions of the bonnet monkey epididymis was electrophoresed on 10% SDS-PAGE, transferred onto a nitrocellulose membrane, and probed with antibody specific to ERα. The blot was stripped and reprobed with an antibody specific to ERβ. Following this, the blot was once again stripped with an antibody specific to actin which served as an internal control for assessing equality of protein loading. Panel F is a graphical representation of this data wherein, the densitometric signal intensities obtained for ERα and ERβ were normalized against that of actin and plotted as percent change of the individual intensities with respect to the caput region. The figure is representative of aleast three independent experiments.