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Table 3 PCR primers, amplicon size and expression ratios of genes between adhesion and normal peritoneal fibroblasts

From: Expression pattern and regulation of genes differ between fibroblasts of adhesion and normal human peritoneum

Transcripts

Primer Sequence (5' to 3')

Amplicon size (base pairs)

Adhesion/Normal Ratio Gene Filter*

Adhesion/Normal Ratio (RT-PCR)**

18S Ribosomal Subunit

Sense

ggaggttcgaagacgatcag

509

(No spot)

0.9

 

Antisense

cgctgagccagtcagtgtag

   

Collagen type IV alpha 2 chain(COL4A2)

Sense

caccatgcccttcctgtact

351

2.6

2.3

 

Antisense

ttgcattcgatgaatggtgt

   

S100 Calcium binding protein A10 (S100A10)

Sense

cacaccaaaatgccatctca

389

2.5

2.1

 

Antisense

cttctatgggggaagctgtg

   

Nidogen 2 (NID2)

Sense

gcttacgaggaggtcaaacg

500

6.8

2.9

 

Antisense

ttcacccggaaggtattcag

   

Transmembrane 4 superfamily member 1 (TM4SF1)

Sense

tcgcggctaatattttgctt

500

3.2

1.9

 

Antisense

gcctccaagcactccattta

   

Chondoitin sulfate proteoglycan 2 (CSPG2)

Sense

gaaccaaattatggggcaga

400

3.3

3.0

 

Antisense

ctcccaatccttcgtcgata

   

Insulin-like growth factor binding protein 3 precursor (IGFBP3)

Sense

gggtaggcacgttgtaggaa

603

-2.8

-2.8

 

Antisese

gtgaggctggctaagaatgc

   

Metallothionine (hMT-Ie)

Sense

cagagggtctctgggtttca

400

4.2

3.3

 

Antisense

gccccatgtcctctcactaa

   
  1. * Average intensity of Adhesion/Normal peritoneal fibroblast gene expression data from patient 1(P1) and 2 (P2) presented in Table 2. Minus (-) sign indicate fold decrease in intensity in the adhesion fibroblasts. ** Ratios of Adhesion/Normal mean values from 4 patients.