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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Expression pattern and regulation of genes differ between fibroblasts of adhesion and normal human peritoneum

Figure 2

Relative abundance of specific mRNA species in the normal and adhesion fibroblasts. Genes differentially expressed between the normal and adhesion fibroblasts, as identified by gene filter experiments, were amplified by the RT-PCR technique at 26 PCR cycle. PCR products (20 μl) were subjected to electrophoresis, stained with fluorescent dye, photographed and optical density determined as described in Methods. A: Representative gel showing amplicons from normal (odd lane numbers) and adhesion (even lane numbers) fibroblasts. Lanes 1 &2, 3 &4; 5 &6; 7 &8; 9 &10 and 11 &12 show RT-PCR products from COL4A2; NID2; CSPG2; S100A10; 18S ribosomal subunit and TM4SF1 mRNA respectively. Lanes 13 &14; 15 &16 and 17 &18 show RT-PCR products from 18S ribosomal subunit, IGFBP3 precursor and MET-1e mRNA respectively. L: Lanes loaded each with 7 μl of 100 bp DNA ladder. The 600 bp band of the ladder is shown by arrow head. B. Histogram showing mean and standard error of mean values of optical densities derived from amplicons of specific genes (x axis) from normal (empty bars) and adhesion (filled bars) fibroblasts isolated from 4 patients as described in Methods. *Significantly different (p < 0.05) between normal and adhesion fibroblasts.

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