Effects of TGFβs on granulosa cell function. Effects of TGF-β1 and TGF-β2 on secretion of progesterone during the last 48 hours of culture (top), content of DNA at the termination of culture (middle) and progesterone concentration per μg of DNA. Values are expressed as the LS mean from 3 separate experiments. The dose of either TGF-β1 or TGF-β2 is indicated along the bottom of the graphs. For each variable, asterisk(s) indicates values that are different from the control (0) value (* p < 0.05; ** p < 0.01, *** p < 0.001). Comparisons were also made between the values obtained for TGF-β1 and TGF-β2 at each dose; however, no significant differences were observed at any dose tested.