Table 1 Oligonucleotide primers and TaqMan probes used for real-time RT-PCR analysis
Gene | Primer or TaqMan probe | Sequence | Position |
|---|---|---|---|
PL-Ala (J02840) | Forward | 5' GCAACATTGGTGGCTAGCAA 3' | 262-281 |
| Â | Reverse | 5' GCCCTCGCCAAACTGTTTATTA 3' | 339-317 |
| Â | Probe | 5' CTATAGGCTCGCCAGGGAAATGTTCAATGA 3' | 285-314 |
PRP-I (J02944) | Forward | 5' CAGACAGGTTTATGAATGCCGC 3' | 458-479 |
| Â | Reverse | 5' CGCAGGCAGTAGAACAGGTTAT 3' | 541-520 |
| Â | Probe | 5' TCCTCTGCATCATCTAGTCACGGAGCTG 3' | 483-511 |
AW464053 | Forward | 5' AATATGCCCAGGGCAAACTG 3' | 296-315 |
| Â | Reverse | 5' TCGGGAGTTTGGAGGGAATT 3' | 368-349 |
| Â | Probe | 5' TCAATGCCATCAAGAGCTGCCACAC 3' | 323-347 |
AW465434 | Forward | 5' ACATCTCCCTGAAAGTGAACCC 3' | 287-308 |
| Â | Reverse | 5' TCCATCCTTGCAGAAGTCTCCT 3' | 369-348 |
| Â | Probe | 5' CCCTGGAAGCTCATCTGCAATGTAAAGC 3' | 316-343 |
AW462349 | Forward | 5' GCGTGGATGGTGTCCTACTTCTA 3' | 216-238 |
| Â | Reverse | 5' GCCACAACGAGAAACAGGAAA 3' | 301-281 |
| Â | Probe | 5' TGTCTGTTTGCCTTTACTGGTGAGCCCT 3' | 240-267 |
AW485575 | Forward | 5' CCTCTGATGAAAGATTGGGAACAG 3' | 195-218 |
| Â | Reverse | 5' AAGTGCCAGAGATCTTGGCCT 3' | 285-265 |
| Â | Probe | 5' TTCTCCAAACCAACCACCACCAGCTG 3' | 230-255 |
GAPDH (U85042) | Forward | 5' AAGGCCATCACCATCTTCCA 3' | 178-197 |
| Â | Reverse | 5' CCACTACATACTCAGCACCAGCAT 3' | 253-230 |
| Â | Probe | 5' AGCGAGATCCTGCCAACATCAAGTGG 3' | 200-225 |