From: Uterine extracellular matrix components are altered during defective decidualization in interleukin-11 receptor α deficient mice
Primer pair
PCR product size (bp)
Annealing temp (C)
No. of cycles
[MgCl2] (mmol/l)
Melting temp of PCR product (C)
COL3A1
522
64
40
3
91.2
BGN
131
58
5
86.3
SPARC
130
60
85.6
NID1
425
63
4
88.8
18S
187
35
86.8