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Table 2 Real-time RT-PCR amplification conditions for each primer pair

From: Uterine extracellular matrix components are altered during defective decidualization in interleukin-11 receptor α deficient mice

Primer pair

PCR product size (bp)

Annealing temp (C)

No. of cycles

[MgCl2] (mmol/l)

Melting temp of PCR product (C)

COL3A1

522

64

40

3

91.2

BGN

131

58

40

5

86.3

SPARC

130

60

40

3

85.6

NID1

425

63

40

4

88.8

18S

187

60

35

4

86.8