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Table 2 Real-time RT-PCR amplification conditions for each primer pair

From: Uterine extracellular matrix components are altered during defective decidualization in interleukin-11 receptor α deficient mice

Primer pair PCR product size (bp) Annealing temp (C) No. of cycles [MgCl2] (mmol/l) Melting temp of PCR product (C)
COL3A1 522 64 40 3 91.2
BGN 131 58 40 5 86.3
SPARC 130 60 40 3 85.6
NID1 425 63 40 4 88.8
18S 187 60 35 4 86.8