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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Methods to find out the expression of activated genes

Figure 5

Model of a microarray. In a single-chip technique reverse transcription from mRNAs to cDNAs is separately carried out for the test and control cell preparations. During the transcription one of the fluorescent dyes (e.g., Cy3 – green and Cy5 – red) are incorporated into the cDNAs of each preparation. A mixture of these two preparations is then hybridised to the corresponding gene-representing sequences on a chip. The activated genes of the control sample exhibit green color, those of the test sample provide red spots, equally bound cDNAs can be visualized by yellow spots, no hybridization remains black.

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