Table 2 Amplicon-specific primer and probe sequences
From: Analysis of Maxi-K alpha subunit splice variants in human myometrium
Primer name | Primer/Probe sequence (5' – 3') | Primer/Probe Tm (°C) |
|---|---|---|
Conserved F | TGCACAAAGAGGTATGTCATCAC | 58.9 |
Conserved R | GTTTGCTGTGGATGGGATGGA | 59.8 |
Conserved Probe | 6F * -CCCACTCGTCGCAGTCCTCCAGCAAGAAGA XT♣ | 68.9 |
132 bp splice F | ACGCTCAAGTACCTGTGGACCGT | 64.2 |
132 bp splice R | TGTGGTTCCAGTTGAGTCACCA | 60.3 |
132 bp Probe | 6F-CTCCAGGGTGGAGTGATTGGCTGTATGTT XTCAC | 71.5 |
87 bp splice F | CATCGCAAGTGATGCCAAAGAA | 58.4 |
87 bp splice R | TCAACTGGCTCGGTCACAAGC | 61.8 |
87 bp Probe | 6F-TTGCAGCTAGATCACGCTATTCCAAAGATCCA XT | 68.9 |
β-actin F | CAACTCCATCATGAAGTGTGAC | 55.8 |
β-actin nested R | GTCAAGAAAGGGTGTAACGCA | 55.4 |
β-actin Probe | 6F-TGGCACCCAGCACAATGAAGATCAAATCA XT | 70.3 |