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Figure 10 | Reproductive Biology and Endocrinology

Figure 10

From: 17beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (Salvelinus alpinus)

Figure 10

Immunoblots of Arctic char plasma proteins from control, E2- (10-7 M), and E2 + F- (10-7 M and 10-5 M) treated fish separated by two-dimensional electrophoresis. 40 μg total protein was separated by isoelectric focusing in the first dimension using a pH gradient 4–7, followed by SDS-PAGE using 8–18% acrylamide gradient. Polyclonal anti-Arctic char vitellogenin was used. Figure show a part of the PVDF-membrane, spots recognized by the vitellogenin antibody are circled.

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