Figure 10
Immunoblots of Arctic char plasma proteins from control, E2- (10-7 M), and E2 + F- (10-7 M and 10-5 M) treated fish separated by two-dimensional electrophoresis. 40 μg total protein was separated by isoelectric focusing in the first dimension using a pH gradient 4–7, followed by SDS-PAGE using 8–18% acrylamide gradient. Polyclonal anti-Arctic char vitellogenin was used. Figure show a part of the PVDF-membrane, spots recognized by the vitellogenin antibody are circled.