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Table 1 Artificial activation of E. coqui eggs

From: In vitro fertilization and artificial activation of eggs of the direct-developing anuran Eleutherodactylus coqui

Egg origin

Treatment

Number cleaving at 10 hours/Number tested (%)

Number cleaving at 16 hours, after being poked at 10 hours/Number tested (%)

Eggs laid in response to hormone treatment

Undisturbed

11/32 (34)

6/8 (75)

 

Poked

28/44 (64)

-

 

A23187

30/36 (83)

-

Oocytes dissected directly from ovisac

Undisturbed

0/40

-

 

A23187

0/40

-

 

Progesterone

0/40

-

 

Progesterone & AA23187

0/40

-