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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella

Figure 3

Immunofluorescence localization of Sp17 and AKAP3. A. Immunofluorescent localization of Sp17 in human spermatozoa. Sp17 is found in the principal piece, middle piece and in scattered patches throughout the head region. The nuclei are DAPI stained. B. Immunofluorescent localization of AKAP3 in human spermatozoa. AKAP3 is located predominantly in the principal piece of the flagella. The nuclei are DAPI stained. C. Co-localization of Sp17 and AKAP3 in human spermatozoa. AKAP3 (green), located in the principal piece, co-localizes with Sp17 in some areas (yellow) of the principal piece but not in others. Sp17 (red) is also located in the middle piece of the tail and head regions. The nuclei are DAPI stained. Scale bar is 20 μm for A, B and C.

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