Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis

Table 4 Effect of glutamine on nuclear maturation and cumulus expansion.

Maturation medium	Nuclear maturation rate % (n° of oocytes)	Cumulus expansion rate % (n° of oocytes)
Control	50% (67) ^a,b	32% (67) ^a
Control + 1 μg/ml eFSH	34% (86) ^a	60% (86) ^b
Control + 2 mM glutamine	36% (59) ^a,b	41% (59) ^a,b
Control + 2 mM glutamine + 1 μg/ml eFSH	34% (80) ^a	51% (80) ^a,b
Control without BSA with 20% FCS	53% (68) ^b	94% (68) ^c

^a,b,c Values with different superscripts are significantly different (p < 0.05).

ISSN: 1477-7827