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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis

Figure 4

Connexin 43 (A), FSH receptor (B) and GAPDH (C) mRNA expression in equine cumulus cells as detected by RT-PCR. Lane 1: 5 compact cumulus cells from medium containing FCS, lane 2: 5 compact cumulus cells from control medium, lane 3: 3 compact cumulus cells from medium containing 5 μg/ml FSH, lane 4: 4 compact cumulus cells from medium containing 1 μg/ml FSH, lane 5: 3 compact cumulus cells from medium containing 0.5 μg/ml FSH, lane 6: 6 compact cumulus cells from medium containing 0.1 μg/ml FSH, lane 7: 6 expanded cumulus cells from medium containing FCS, lane 8: 3 expanded cumulus cells from control medium, lane 9: 3 expanded cumulus cells from medium containing 5 μg/ml FSH, lane 10: 3 expanded cumulus cells from medium containing 1 μg/ml FSH, lane 11: 5 expanded cumulus cells from medium containing 0.5 μg/ml FSH, lane 12: 9 expanded cumulus cells from medium containing 0.1 μg/ml FSH, lane 13: H2O.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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