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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis

Figure 3

Connexin 43 (A), cyclooxygenase 2 (B) and GAPDH (C) mRNA expression in equine cumulus cells as detected by RT-PCR. Lanes 1, 2 and 3: 3 pools of 2 cumulus cells pellets from control medium, lanes 4, 5 and 6: 3 pools of 2 pellets from medium containing precursors, lanes 7, 8 and 9: 3 pools of 2 pellets from medium containing precursors and FSH, lanes 10, 11 and 12: 3 pools of 2 pellets from medium containing FCS, lane 13: H2O.

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