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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis

Figure 2

Connexin 43 (A), cyclooxygenase 2 (B) and GAPDH (C) mRNA expression in equine cumulus cells as detected by RT-PCR. Lanes 1 and 2: 2 pools of 4 cumulus cells pellets from medium containing FCS, lanes 3 and 4: 2 pools of 4 pellets from control medium, lanes 5 and 6: 2 pools of 4 pellets from medium containing FSH, lanes 7 and 8: 2 pools of 2 pellets from in vivo mature control, lanes 9 and 10: 2 pools of 3 pellets from immature control, lane 11: H2O.

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