Figure 4

A schematic presentation of Schwann cell markers expressed during various developmental stages. These markers are tentatively divided into 3 groups. Group 1 are genes and proteins, including p75, GFAP and SCIP (shown in blue), that express temporally during the development between immature Schwann cells and matured, myelinating cells. Group 2 markers include S100, O4 and GC (shown in red) that express during the progenitor or immature stages and continue into matured, myelinating Schwann cells. Group 3 markers include myelinating genes and proteins such as MBP and PLP (shown in green) that express only in the mature stage of myelinating Schwann cells. Monkey ES cell-derived glial phenotypes formed cell bundles and aggregates that expressed markers of all 3 groups (shown in immunofluorescence green images; nuclei were counter-stained with DAPI in blue color), except GC, which expressed in some, but not all, samples (image not shown). The insert shows gene markers detected by RT-PCR in these monkey ES cell-derived glial phenotypes.