Figure 3
From: Derivation and characterization of monkey embryonic stem cells
Differentiation of monkey ES cells into glial phenotypes. Panel A, morphology in culture; Panel B, aggregated glial cell bundles and spheres cultured on laminin-coated glass coverslips; Panel C, expression of Schwann cell markers S100 and GFAP; Panel D, expression of Schwann cell markers GFAP and p75 (NGFR); Panel E, expression of myelin protein MBP and Schwann cell marker GC; Panel F, expression of myelin protein PLP and neuronal marker MAP2C. Panel G shows the temporal pattern of Schwann cell molecular signatures during development and a mixture of glial phenotypes derived from monkey ES cells expressing these Schwann cell markers as determined by immuocytochemistry (panel H) and RT-PCR (panel I).