Skip to main content

Table 1 Procedural steps in producing a genetically modified rhesus macaque

From: Creation of non-human primate neurogenetic disease models by gene targeting and nuclear transfer

1. Isolate isogenic genomic fragments using long and accurate PCR
2. Construct targeting construct
3. Transfect TERT life-span extended cells with targeting vector
4. Select and identify targeted clones
5. Remove TERT
6. Nuclear transfer
7. Embryo transfer
8. Analysis of genetically modified NHP