Table 1 Procedural steps in producing a genetically modified rhesus macaque
From: Creation of non-human primate neurogenetic disease models by gene targeting and nuclear transfer
1. | Isolate isogenic genomic fragments using long and accurate PCR |
2. | Construct targeting construct |
3. | Transfect TERT life-span extended cells with targeting vector |
4. | Select and identify targeted clones |
5. | Remove TERT |
6. | Nuclear transfer |
7. | Embryo transfer |
8. | Analysis of genetically modified NHP |