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Table 2 Binding characteristics of recombinant human relaxin at putative binding sites and intracellular signals affected.

From: Biology of primate relaxin: A paracrine signal in early pregnancy?

Cell Type

Kd (nM)/# binding sites per cell *

Intracellular signal or Biological Effect

Reference

Human THP-1 Monocytes

0.102/275

↑ cAMP

↑ Extracellular acidification

[195]

Human Uterine Fibroblast

4.4 ± 1.7/3220 ± 557

↑ Tyrosine phosphorylation

[196]

Human Uterine Epithelial Cells

0.44/1082 ± 62

NA

[28]

Human Endometrial Stromal Cells

0.150/963–1000

↑ p42/44 MAPK activity

↑ Activation of CREB

↑ VEGF expression

↑ cAMP

↑ Prolactin gene expression

[103]

[119]

[197]

Human Sperm

0.650/NA

↑ Sperm motility

↑ Acrosome reaction

[198]

Human Granulosa Cells

NA

↑ Release of calcium from intracellular stores

[199]

  1. cAMP = cyclic adenosine monophosphate; CREB = cAMP response element binding protein; MAPK, mitogen-activated protein kinase; NA = data not available; VEGF = vascular endothelial growth factor. The asterisk (*) indicates that reported estimates for Kd values may be different due to tissue and radioligand differences, or both.