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Table 2 Antibodies used in immunocytochemistry

From: Expression of connexins in human preimplantation embryos in vitro

Antigen

Antibody Isotype

Dilution

Secondary Antibody

Dilution

Cx26

Rabbit polyclonal [34]

1:1000

Alexa fluor 546, goat anti rabbit IgG

1:125

Cx31

Rabbit polyclonal [35]

1:300

Alexa fluor 546, goat anti rabbit IgG

1:125

Cx40

Rabbit polyclonal [36]

1:100

Alexa fluor 546, goat anti rabbit IgG

1:125

Cx43

Mouse IgG (Zymed)

1:500

Alexa fluor 488, goat anti mouse IgG

1:125

Cx45

Rabbit polyclonal [35]

1:500

Alexa fluor 546, goat anti rabbit IgG

1: 125