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Table 2 Antibodies used in immunocytochemistry

From: Expression of connexins in human preimplantation embryos in vitro

Antigen Antibody Isotype Dilution Secondary Antibody Dilution
Cx26 Rabbit polyclonal [34] 1:1000 Alexa fluor 546, goat anti rabbit IgG 1:125
Cx31 Rabbit polyclonal [35] 1:300 Alexa fluor 546, goat anti rabbit IgG 1:125
Cx40 Rabbit polyclonal [36] 1:100 Alexa fluor 546, goat anti rabbit IgG 1:125
Cx43 Mouse IgG (Zymed) 1:500 Alexa fluor 488, goat anti mouse IgG 1:125
Cx45 Rabbit polyclonal [35] 1:500 Alexa fluor 546, goat anti rabbit IgG 1: 125