Table 1 Gene specific amplification primers
From: Expression of connexins in human preimplantation embryos in vitro
Target Gene | Primer pair sequences (5' – 3') | Accession number | Position in sequence | Fragment Size (bp) | Annealing Temp (\r C) |
|---|---|---|---|---|---|
β actin | GACAGCAGTCGGTTGGACC | M10277 | 3163–3179 | 387 | 62 |
|  | CAGGTAAGCCCTGGCTGC |  | 3549–3532 |  |  |
Cx26 (a) | GTTTAACGCATTGCCCAGTT | M86849 | 874–893 | 174 | 62 |
|  | TGTGGCATCTGGAGTTTCAC |  | 1047–1028 |  |  |
Cx26 (b) | AGGCCTGTCCAACACATCTC |  | 1760–1779 | 244 | 64 |
|  | AGGGGGTAAGCCAAACAAAC |  | 2003–1984 |  |  |
Cx31 | TGCAGTGGAGAGGAGGTCTT | BC012918 | 1454–1473 | 184 | 66 |
|  | CAGTTGTGGGGAGGAAGATG |  | 1637–1618 |  |  |
Cx32 (a) | GGGTACAAGAGATGGGATGC | BC039198 | 1200–1219 | 202 | 64 |
|  | GCCAGCAAGCACTATTCCTC |  | 1401–1382 |  |  |
Cx32 (b) | CCCTGGTTTTCTGGAGTCAC | NM_000166 | 1273–1292 | 206 | 62 |
|  | CCCTGCTCCAACTTATCTGC |  | 1478–1459 |  |  |
Cx40 (a) | TTGCAACCTTTCCTTCTGCT | BC013313 | 1878–1897 | 180 | 64 |
|  | GTGAACAGCCAAGGGAGAAA |  | 2057–2038 |  |  |
Cx40 (b) | CCCTGCTAGGGAGTCACTGT |  | 1929–1948 | 201 | 62 |
|  | CTGGTCAGGGTTCGAGAGAG |  | 2129–2110 |  |  |
Cx43 | CTGACATGCATGCAAGAAGAA | BC026329 | 2802–2782 | 217 | 64 |
|  | TCTTTTGGAGTGACCAGCAA |  | 2586–2605 |  |  |
Cx45 | AGATCAGGATGGCTCAGGAA | U03493 | 1022–1041 | 155 | 64 |
|  | GTTCTTCCCATCCCCTGATT |  | 1176–1157 |  |  |