Figure 6
From: Origin of germ cells and formation of new primary follicles in adult human ovaries
Double color immunohistochemistry of the oocyte-nest assembly. A) Occupied "bird's" nest type indicates a half way oocyte-nest assembly. B) An earlier stage of assembly shows the oocyte embraced by nest trunks, resembling an octopus. Immunostaining with CK monoclonal antibody, clone MNF116 (recognizing CK5, 6, 8, 17) – first sequence visualized with diaminobenzidine (brown color), and with HSPZ (zona pellucida) polyclonal antibody – second sequence visualized with SG (blue color). No hematoxylin counterstain.